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BDI RESEARCH PUBLICATIONS

04.12



Robert Burger, a postgraduate student in Prof. Jens Ducrée's research group, BDI has had great recent publication success. He had a paper published in "Lab on a Chip" and another accepted for publication in "Expert Review of Molecular Diagnostics".

In the review paper "Handling and analysis of cells and bioparticles on centrifugal microfluidic platforms" which was recently accepted in Expert Review of Molecular Diagnostics the drawbacks of typical cell handling operations such as sorting, counting, trapping and assaying on lab-on-a-disc systems were critically surveyed and analysed. Based on the pioneering work covered in the article, BDI researchers believe that centrifugal cell analysis platforms bear a huge potential for next-generation point-of-care devices and companion diagnostics.

In an additional publication, BDI research on "Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform" has recently been published in the journal Lab on a Chip. This article describes novel technology, involving particles which sediment into an array of V-shaped cups under stagnant, i.e. no-flow conditions; that are merely controlled by the centrifugal force. In the unique absence of flow through the array during sedimentation, the capturing efficiency has been shown to be close to 100 %, which is much higher than what is typically achieved by pressure-driven systems. By scale matching the V-cups to the size of the particles, a very narrow, even single occupancy distribution can be achieved which greatly facilitates counting and assay readout. The article also demonstrates the application of this technology to perform bead-based immunoassays. BDI researchers are planning to apply this lab-on-a-disc platform to capture cells and demonstrate operations such as cell counting. Potential applications include white blood cell counts in the BDI-2 strand IMP1 or counting of CD4 cells to monitor HIV patients in the EI-funded BDI-2 project CD4CD4.